ABSTRACT
Objetivou-se com este trabalho avaliar a diversidade genética do gene HSP-70.1 e associar os polimorfismos encontrados com a performance de vacas leiteiras das raças Holandesa, Girolando (5/8H-G) e Sindi criadas em região do semiárido brasileiro. Os polimorfismos foram identificados e avaliados pela técnica de PCR-RFLP, usando-se a enzima de restrição EcoRII. Avaliou-se a variabilidade genética por meio do índice de diversidade padrão e da análise de variância molecular (AMOVA). Os polimorfismos identificados foram avaliados sobre as características de produção de leite. Foram identificados sete alelos, os quais demonstraram que houve polimorfismo para a região gênica analisada, e alguns alelos foram compartilhados entre os rebanhos. As raças bovinas Holandesa e Sindi foram similares geneticamente para o gene analisado. A AMOVA demonstrou que há variação genética entre os rebanhos e dentro deles, com a maior parte da variação ocorrendo dentro dos rebanhos para todos os grupos avaliados. Houve efeito dos alelos identificados sobre a produção de leite dos rebanhos das raças Holandesa (P<0,0001) e Girolando (P<0,0117). O gene HSP-70.1 foi polimórfico na população de bovinos leiteiros estudada, sendo, portanto, um marcador molecular promissor para avaliar a produção de leite de raças criadas em região semiárida.(AU)
The objective of this work was to evaluate the genetic diversity of the HSP-70.1 gene and to associate the polymorphisms found with the performance of Holstein, Girolando (5/8H-G) and Sindi dairy cows raised in region of the Brazilian semiarid. Polymorphisms were identified and evaluated using the PCR-RFLP technique using the EcoRII restriction enzyme. Genetic variability was evaluated using the standard diversity index and molecular variance analysis (AMOVA). The identified polymorphisms were evaluated on the characteristics of milk production. They were identified from the seven alleles, demonstrating that there was polymorphism for the analyzed gene region and some alleles were shared among the herds. The Holstein and Sindi bovine breeds were genetically like the analyzed gene. AMOVA demonstrated that there is genetic variation between and within the herds, with most of the variation occurring within the herds for all groups evaluated. There was effect of the alleles identified on the production of milk herds of Holstein and (P<0.0001) Girolando (P<0.0117) breeds. The HSP-70.1 gene was polymorphic in the population of dairy cattle studied, and therefore a promising molecular marker to evaluate milk production of breeds created in semiarid regions.(AU)
Subject(s)
Animals , Cattle , HSP70 Heat-Shock Proteins/analysis , HSP70 Heat-Shock Proteins/genetics , Heat Stress Disorders/veterinary , Polymerase Chain Reaction/veterinary , Analysis of Variance , Semi-Arid Zone , ThermotoleranceABSTRACT
Abstract Objetive: Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) improved symptoms and increased survival and quality of life in patients with coronary artery disease. However, it should be the main cause of a complex organic systemic inflammatory response that greatly contributes to several postoperative adverse effects. Methods: We aimed to evaluate heat-shock protein 70 (HSP 70) expression as a morbimortality predictor in patients with preserved ventricular function undergoing coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) and to determine their association with the lactate as a marker of tissue hypoperfusion and the EuroSCORE risk score. This is a prospective, observational study including 46 patients and occurring between May and July 2016. Patients without ventricular dysfunction undergoing myocardial revascularization with extracorporeal circulation were included. They were divided into (1) complicated and (2) uncomplicated postoperative evolution groups. EuroSCORE, lactate levels, and HSP 70 expression and their correlations were determined. Results: Statistical analysis showed that the group with complicated evolution had higher EuroSCORE values than the other group. HSP 70 protein levels were significantly increased in the group with uncomplicated evolution and showed similar results. According to our results, HSP family proteins may be independent predictors of uncomplicated evolution in patients without ventricular dysfunction undergoing CABG with CPB. Conclusion: HSP 70 should be a good discriminator and protection marker for complications in cardiac surgery.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Cardiopulmonary Bypass/mortality , Coronary Artery Bypass/mortality , Risk Assessment/methods , HSP70 Heat-Shock Proteins/analysis , Lactic Acid/blood , Preoperative Period , Postoperative Complications/etiology , Biomarkers/analysis , Cardiopulmonary Bypass/methods , Logistic Models , Blotting, Western , Coronary Artery Bypass/methods , Sensitivity and Specificity , Statistics, Nonparametric , Myocardium/pathologyABSTRACT
Background: Heat shock proteins are a highly conserved group of protective cellular proteins whose synthesis is increased in response to a variety of environmental or pathophysiological stresses. Heat shock proteins are useful biomarkers for carcinogenesis in tissues and signal the degree of differentiation and the aggressiveness of cancers. Regulation of heat shock protein 70 (HSP70) expression in oral submucous fibrosis is not known much, and the aim of this study was to evaluate HSP70 expression in oral submucous fibrosis and oral squamous cell carcinoma by immunohistochemical method and to understand the role of HSP70 in tumorigenesis. Materials and Methods: Immunohistochemical method was used to detect HSP70 expression in normal oral mucosa, oral submucous fibrosis (n=30) and oral squamous cell carcinoma (n=20). HSP70 immunoreactivity was correlated with histological and clinicopathological features. Results: A significant increase in expression of HSP70 was observed (P<0.000) as the tissue progressed from oral submucous fibrosis towards oral squamous cell carcinoma. Conclusion: HSP70 is synthesized upon stress situations arising in cells of all living organisms. Expression of HSP70 indicates that stress plays an important role as a predisposing factor in oral submucous fibrosis and its subsequent progression to oral squamous cell carcinoma.
Subject(s)
Adolescent , Adult , Age Factors , Aged , Biomarkers/analysis , Carcinoma, Squamous Cell/pathology , Cell Transformation, Neoplastic/pathology , Child , Child, Preschool , Disease Progression , Gingiva/anatomy & histology , HSP70 Heat-Shock Proteins/analysis , Humans , Immunohistochemistry , Middle Aged , Mouth Mucosa/pathology , Mouth Neoplasms/pathology , Oral Submucous Fibrosis/pathology , Precancerous Conditions/pathology , Young AdultABSTRACT
OBJETIVOS: Em modelos animais de epilepsia, heat shock protein 70 (HSP70) tem sua expressão proporcional à intensidade de crises. A HSP90, dentre diversas ações, regula a sintase neuronal do óxido nítrico e proteínas do citoesqueleto. Devido ao provável papel protetor de HSP70 e à relação de HSP90 com proteínas envolvidas na epileptogênese, decidimos investigar a expressão imunohistoquímica destas proteínas na epilepsia do lobo temporal mesial (ELTM). MÉTODOS: Hipocampos de pacientes ELTM fármaco-resistentes foram obtidos durante o procedimento cirúrgico e hipocampos controle foram obtidos de necrópsias. Os espécimes obtidos foram tratados igualmente e submetidos a imunohistoquímica. Medidas de imuno-reatividade positiva foram obtidas com o software ImageJ. RESULTADOS: Nossas medidas mostraram menor expressão de HSP70 e HSP90 no hipocampo de pacientes epilépticos do que nos controles em praticamente todas as regiões do hipocampo. Para HSP70 as diferenças significativas foram encontradas na região subicular e para HSP90 em todas, exceto fascia dentata e subículo. CONCLUSÃO: Diferente dos achados em modelos animais, nossos resultados indicam que crises crônicas nos pacientes ELTM não são estímulo suficiente para ativação exacerbada de HSP70 e HSP90. Condições inerentes à ELTM podem ser determinantes desta menor expressão. Ainda, nossos achados sugerem que a baixa expressão de HSPs pode estar relacionada a manutenção das crises.
OBJECTIVE: In animal models of epilepsy, heat shock protein 70 (HSP70) has its expression proportional to seizure severity. Among several functions on biological systems, HSP90 regulates nitric oxide synthase and cytoskeletal proteins. Due to the plausible protective role of HSP70 and the relationship of HSP90 with proteins involved in epileptogenesis, we looked at HSP70 and 90 immunohistochemical expression in temporal lobe epilepsy (TLE). METHODS: Hippocampi were obtained from medically intractable TLE patients and control hippocampi were from necropsy cases. Specimens were equally treated and submitted to imunohistochemistry to HSP70 and HSP90. Positive immunoreactivity was estimated using the software ImageJ. RESULTS: Our results showed significant lower expression of HSP70 and HSP90 in epileptic patients when compared to controls in almost all hippocampal regions. To HSP70 subicular region exhibited significant difference and to HSP90 all regions, except fascia dentata and subiculum. CONCLUSION: Unlike the reports in animal models the present results indicate that chronic seizures in TLE patients are not sufficient to induce HSP70 and HSP90 activation. Typical attributes inherent to TLE condition may be determinants of low HSP expression. In Addition, our results suggest that low expression of HSPs in epileptic groups may be related to seizure maintenance.
Subject(s)
Humans , Immunohistochemistry/instrumentation , HSP90 Heat-Shock Proteins/analysis , HSP70 Heat-Shock Proteins/analysis , Epilepsy, Temporal LobeABSTRACT
BACKGROUND & OBJECTIVE: Myocardial hypofunction could lead to the derangement of brain functions. The expression of c-fos and heat shock protein (hsp) genes was recognized as markers of neural cell injury. We under took this study to investigate the influence of myocardial ischaemia and reperfusion (I/R) on these molecular events in the rat brain tissue and changes in mean arterial blood pressure (MAP) and the cerebral blood flow velocity (CBFV), to understand the basis of cerebral pathology following cardiac ischaemia and reperfusion. METHODS: Healthy rats (n=42) were randomly allocated into seven groups: sham-operated (A); myocardial ischaemia for 15 min, followed by 0.5, 1, 2, 4 and 6 h of reperfusion respectively (B2-B6) and hypovolemia at medium level (C). I/R rat models were established by ligating the anterior branch of the left coronary artery. Expressions of the c-fos, hsp70, hsp27 and hsp90 genes in the cerebrum, cerebellum, medullaoblongata and hippocampus, were studied with immunohistochemistry and in situ hybridization. The MAP and CBFV of the rats were also measured. RESULTS: The expressions of c-fos and HSP70 in brain tissue increased after myocardial ischaemia/ reperfusion, with the strongest signal appearing in the hippocampus and cerebral cortex, while labeling for HSP27 and HSP90 alpha were not detectable in any of the experimental groups. The expressions of c-fos mRNA and hsp70 mRNA shared the similar characteristics with their encoding proteins. MAP, reflection of cardiac function and cerebral blood flow decreased following cardiac ischaemia and reperfusion. INTERPRETATION & CONCLUSION: Our findings suggested that the brain damages occurred from the early phase of myocardial I/R. The exact mechanism of cerebral tissue injuries induced by myocardial I/R is not known. Further studies need to be done to throw light on these aspects.